High-accuracy in vitro assessments of compounds for neural and cardiac applications, tailored to user needs
Contract Research
We design test plans in accordance with the client's needs and conduct compound tests with high accuracy.
Data Analysis
Data analysis of MEA, Ca2+ transients, Imaging, EEG/ECoG, and correlation analyses with multiple assays and in vivo studies. We also analyze data acquired by clients.
Consulting
We provide technical consulting on cell culture, MEA assay methods, etc., and corporate consulting based on international trends in compound evaluation.
Collaborative Research and Development
We develop culture, measurement, and data analysis techniques and construct new compound evaluation systems in accordance with the client's needs.
Screening
Phenotypic screening based on similarity to known compounds and mechanism of action.
Human and Rodent models
Human iPSC-derived neurons and glial cells, Human iPSC-derived sensory and motor neurons, Human Brain organoids, Rodent cortical and hippocampal neurons, Rodent DRG neurons, and Human iPSC-derived cardiomyocytes.
*Includes various disease models.
Based on our experience in culturing more than 100 types of abundant human iPS neuronal cells and evaluating more than 1,000 compounds, we propose in a custom approach tailor-made to your CNS, PNS, and Heart drug discovery needs.
We are also available for evaluation using client's cells and optimization study of culture conditions.
Assays
VitroVo offers a wide range of electrophysiological assays on various in vitro neural networks, brain organoids, acute brain slices, and cardiomyocytes using micro-electrode array (MEA) technology. We will select the appropriate MEA system for your study from various MEA systems.
Ca2+imaging, Immunohistochemistry/
We select a cell/tissue model that meets the client's needs and construct an experimental design, combining multiple assays as needed.
Using our data analysis techniques, we provide results for predicting the toxicity, efficacy, and mechanism of action of the compound.
HD-MEA
High Density (HD) microelectrode arrays, composed of tens to hundreds of thousands of microelectrodes, are utilized to perform electrophysiological measurements on a variety of in vitro neural networks, brain organoids, acute brain slices, and different iPSC-derived cardiomyocytes. Assessments of synaptic function, axonal conduction, network connections, and inter-tissue propagation patterns are achieved through single-cell activity analysis. By employing novel parameters, cutting-edge analyses are conducted that delve into underlying mechanisms.
Data Analysis
Experienced professionals will provide predictive results on the safety and mechanisms of action of compounds. Correlative analyses with multiple assays, as well as correlations with in vivo studies, will also be conducted.
We possess unique multivariate analysis techniques, AI technologies for waveform data analysis, and AI imaging analysis capabilities. Additionally, we excel in analyzing low-frequency components in brain organoid and astrocyte
We also analyze MEA data, Ca2+imaging data, and EEG/ECoG data acquired by clients.
Micro-physiological system (MPS)
We conduct compound evaluations using micro-physiological system (MPS) technology. Our approach involves high-throughput cultivation suitable for compound assessment and uses heterotypic cell-coupling models that mimic biological interactions across different regions and tissues to evaluate compounds. We predict the toxicity, efficacy, and mechanisms of action of compounds through AI analysis of measurement data and multivariate analysis.